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CHEM108: Analytical Chemistry

Unit 3: Atomic Spectrocsopy   This unit covers atomic spectroscopy.  Atomic spectroscopy is an extension of spectrochemical methods.  In IR,there is not enough energy to excite electrons as in fluorescence and phosphorescence,but there is enough to produce changes in vibrational and rotation modes.  Fluorescence and phosphorescence occurs when electrons within a structure are excited by electromagnetic energy (absorption) and then allowed to relax to their ground state.  The energy released from this relaxation process can then be detected and is related to molecular structure.  This unit also discusses turbidimetry and nephelometry, which does not involve the exchange of energy but relates directly to the scattering of light. 

Unit 3 Time Advisory
This unit will take approximately 6.5 hours to complete.

☐    Subunit 3.1: 1.5 hours

☐    Subunit 3.2: 4.5 hours

☐    Subunit 3.3: 0.5 hours

Unit3 Learning Outcomes
Upon successful completion of this unit, the student will be able to:

  • Explain the theory of atomic absorption and atomic emission.
  • Identify components and design of fluorescence and phosphorescence instruments.
  • Define line spectra and describe how it arises.
  • Demonstrate an understanding of turbidimetry and nephelometry.

3.1 Atomic Absorption (AA)   - Reading: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10D: Atomic Absorption Spectroscopy” Link: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10D: Atomic Absorption Spectroscopy” (PDF)

 Instructions: Click on the link to Chapter 10 to open the PDF. 
Read section 10D, starting on page 599 of the text.     

 Terms of Use: <span href="http://purl.org/dc/dcmitype/Text"
rel="dc:type">Analytical Chemistry 2.0</span> by [David
Harvey](http://web.me.com/dtharvey1213/Analytical_Chemistry_2.0/Welcome.html) is
licensed under a [Creative Commons
Attribution-NonCommercial-ShareAlike 3.0 Unported
License](http://creativecommons.org/licenses/by-nc-sa/3.0/). The
original version can be
found [here](http://www.asdlib.org/onlineArticles/ecourseware/Analytical%20Chemistry%202.0/Text_Files.html). 
  • Web Media: YouTube: Amy Hrdina’s “Atomic Absorption Spectrophotometry” Link: YouTube: Amy Hrdina’s “Atomic Absorption Spectrophotometry” (YouTube)

    Instructions: Watch this brief video (runtime: 2:37 minutes).  This video demonstrates the laboratory technique used for flame atomic absorption spectroscopy.

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

3.2 Emission Spectroscopy   - Reading: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10E: Emission Spectroscopy” Link: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10E: Emission Spectroscopy” (PDF)

 Instructions: Click on the link to Chapter 10 to open the PDF. 
Read section 10E, starting on page 612 of the text.     

 Terms of Use: <span href="http://purl.org/dc/dcmitype/Text"
rel="dc:type">Analytical Chemistry 2.0</span> by [David
Harvey](http://web.me.com/dtharvey1213/Analytical_Chemistry_2.0/Welcome.html) is
licensed under a [Creative Commons
Attribution-NonCommercial-ShareAlike 3.0 Unported
License](http://creativecommons.org/licenses/by-nc-sa/3.0/). The
original version can be
found [here](http://www.asdlib.org/onlineArticles/ecourseware/Analytical%20Chemistry%202.0/Text_Files.html). 

3.2.1 Fluorescence and Phosphorescence   - Reading: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10F: Photoluminescence Spectroscopy” Link: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10F: Photoluminescence Spectroscopy” (PDF)

 Instructions: Click on the link to Chapter 10 to open the PDF. 
Read section 10F, starting on page 613 of the text.     

 Terms of Use: <span href="http://purl.org/dc/dcmitype/Text"
rel="dc:type">Analytical Chemistry 2.0</span> by [David
Harvey](http://web.me.com/dtharvey1213/Analytical_Chemistry_2.0/Welcome.html) is
licensed under a [Creative Commons
Attribution-NonCommercial-ShareAlike 3.0 Unported
License](http://creativecommons.org/licenses/by-nc-sa/3.0/). The
original version can be
found [here](http://www.asdlib.org/onlineArticles/ecourseware/Analytical%20Chemistry%202.0/Text_Files.html). 
  • Reading: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Theoretical Principles” Link: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Theoretical Principles” (HTML)

    Instructions: Read the entire webpage.  This material explains the process of emission and how it is utilized in analysis of chemical compounds.

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Assessment: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Theoretical Principles Quiz” Link: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Theoretical Principles Quiz”(HTML)

    Instructions: Complete the quiz, and then check your answers by clicking on the button at the bottom of the page. 

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Reading: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Instrumentation” Link: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Instrumentation” (HTML)

    Instructions: Read the entire webpage.  This material describes the components and setup of fluorescence and phosphorescence spectrophotometers. 

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Assessment: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Instrumentation Quiz” Link: Sheffield Hallam University’s “UV-Vis Luminescence Spectroscopy – Instrumentation Quiz”(HTML)

    Instructions: Complete the quiz, and then check your answers by clicking on the button at the bottom of the page. 

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

3.2.2 Atomic Emission Spectroscopy   - Reading: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10G: Atomic Emission Spectroscopy” Link: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10G: Atomic Emission Spectroscopy” (PDF)

 Instructions: Click on the link to Chapter 10 to open the PDF. 
Read section 10G, starting on page 626 of the text.     

 Terms of Use: <span href="http://purl.org/dc/dcmitype/Text"
rel="dc:type">Analytical Chemistry 2.0</span> by [David
Harvey](http://web.me.com/dtharvey1213/Analytical_Chemistry_2.0/Welcome.html) is
licensed under a [Creative Commons
Attribution-NonCommercial-ShareAlike 3.0 Unported
License](http://creativecommons.org/licenses/by-nc-sa/3.0/). The
original version can be
found [here](http://www.asdlib.org/onlineArticles/ecourseware/Analytical%20Chemistry%202.0/Text_Files.html). 
  • Web Media: You Tube: Amrita University’s “Emission Spectra Simulation” Link: You Tube: Amrita University’s “Emission Spectra Simulation” (YouTube)

    Instructions: Watch this short video (runtime: 2:41 minutes).  This video demonstrates atomic emission of various compounds.

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

3.3 Turbidimetry and Nephelometry   - Reading: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10H: Spectroscopy Based on Scattering” Link: David Harvey’s Analytical Chemistry 2.0: “Chapter 10, Section 10H: Spectroscopy Based on Scattering” (PDF)

 Instructions:  Click on the link to Chapter 10 to open the PDF. 
Read section 10H, starting on page 636 of the text.     

 Terms of Use: <span href="http://purl.org/dc/dcmitype/Text"
rel="dc:type">Analytical Chemistry 2.0</span> by [David
Harvey](http://web.me.com/dtharvey1213/Analytical_Chemistry_2.0/Welcome.html) is
licensed under a [Creative Commons
Attribution-NonCommercial-ShareAlike 3.0 Unported
License](http://creativecommons.org/licenses/by-nc-sa/3.0/). The
original version can be
found [here](http://www.asdlib.org/onlineArticles/ecourseware/Analytical%20Chemistry%202.0/Text_Files.html).