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BIO304L: Human Physiology Lab

Unit 12: Reproduction   Using sea urchins, the lab in this unit demonstrates the release of egg and sperm cells following an injection of KCL.  You will also learn about the process of fertilization: using a microscope, you will observe how only one sperm cell fertilizes an egg cell.  

Unit 12 Time Advisory
This unit will take approximately 4 hours to complete.

  • Web Media: YouTube: ljin76: “Sea Urchin Fertilization,” amesberto: “In Vitro Fertilization,” joogla: “Sea Urchin Egg Extraction,” and mleguia: “Sea Urchin Fertilization” Link: YouTube: ljin76: “Sea Urchin Fertilization,” (YouTube) amesberto: “In Vitro Fertilization,” (YouTube) joogla: “Sea Urchin Egg Extraction,” (YouTube) and mleguia: “Sea Urchin Fertilization” (YouTube)
     
    Instructions: The first video clip (about 2 minutes) reviews sea urchin fertilization.  The second clip (1 minute) briefly overviews a sea urchin experiment involving the extraction of sperm and egg cells and fertilization.  “Sea Urchin Egg Extraction” (about 6 minutes) demonstrates how to inject KCL into a sea urchin in order to promote the release of eggs.  The last video (10 seconds) depicts how sperm “attack” and consequently fertilize an egg.  
     
    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Activity: Stanford University: “Sea Urchin Embryology” Link: Stanford University: “Sea Urchin Embryology” (HTML)
     
    Instructions: Please review this lab to learn about the extraction of egg and sperm cells from sea urchins using KCL injections.  You will also learn about egg fertilization using a sperm suspension and a microscope.  The microscopic image, which depicts the fertilization of an egg by a sperm cell, is a still from the “Sea Urchin Fertilization” short video linked above. 
     
    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.