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BIO304L: Human Physiology Lab

Unit 11: Digestion   This section reviews laboratory tests demonstrating the digestion processes of carbohydrates (starch, glucose, sucrose), proteins (e.g. egg whites), and lipids (e.g. vegetable oil).  You will also learn about the roles that the digestive enzymes and the pH play in these processes.  

Unit 11 Time Advisory
This unit will take approximately 6 hours to complete.

11.1 Digestion: Overview   - Web Media: BozemanBiology: Paul Andersen's "The Digestive System" Link: BozemanBiology: Paul Andersen's "The Digestive System" (YouTube)
 
Instructions: Please watch this video to learn about the digestive process and the enzymes involved.
 
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11.2 Digestion: Carbohydrates – Starch, Glucose, Sucrose   - Web Media: YouTube: annemifamile: “GCSE Biology Practical Investigation Amylase Starch” and hartonline: “Benedict Test for Reducing Sugars” and “Benedict Test for Non-Reducing Sugars” Links: YouTube: sam2064: “GCSE Biology Practical Investigation Amylase Starch,” (YouTube) and hartonline: “Benedict Test for Reducing Sugars” (YouTube) and “Benedict Test for Non-Reducing Sugars” (YouTube)
 
Instructions: Please watch the first video (about 3.5 minutes) to learn about the digestion of starch using iodine and amylase.  The second video (about 1.5 minutes) demonstrates the Benedict test used to test for glucose, and the third video clip (2.50 minutes) presents a test for sucrose in the presence of HCl or NaOH and Benedict’s reagent. 
 
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  • Activity: The Woodrow Wilson National Fellowship Foundation: “The Effect of pH on Enzyme Activity—Salivary Amylase and Starch Digestion” and The University of Manitoba: Biochemistry: The Molecules of Life: “Carbohydrates” Links: The Woodrow Wilson National Fellowship Foundation: “The Effect of pH on Enzyme Activity—Salivary Amylase and Starch Digestion” (HTML) and hartONline’s “Starch Test” (YouTube)

    Instructions: Via the first link, please read through the experiment to learn about the effect of pH on amylase and the procedure applied to test for starch.  In the second link, work through exercises 1, 2, and 3 for an introduction to Benedict’s and Iodine reagents, including the colors they produce in presence of glucose, sucrose, and starch.   

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11.3 Digestion: Protein   - Web Media: YouTube: JoseSantana123123: “Biuret and Protein Test” Link: YouTube: JoseSantana123123: “Biuret and Protein Test” (YouTube)
 
Instructions: Please watch the video (about 1.5 minutes) for a demonstration of a Biuret test, a procedure which tests for proteins. 
 
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  • Assessment: Biology.resources.com: Experiment Enzymes: “Experiment 10: The Effect of Pepsin on Egg White Suspension” Link: Biology.resources.com: Experiment Enzymes: “Experiment 10: The Effect of Pepsin on Egg White Suspension” (Microsoft Word)

    Instructions: To open the document, click on the tenth lab under “Enzymes.” Please read through the experiment and answer the questions based on the information from this experiment and the “Biuret and Protein Test” video clip. Then check your answers by scrolling down to the last page.

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11.4 Digestion: Lipids   - Web Media: YouTube: dlwestphallen: “Fat Test” Link: YouTube: dlwestphallen: “Fat Test” (YouTube)
 
Instructions: Please watch the video (about 2 minutes) to observe how alcohol and water can test for the presence of fats. 
 
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