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BIO304L: Human Physiology Lab

Unit 2: Enzyme   This section will review the role of enzymes in catalyzing chemical reactions in our bodies.  In this lab, you will learn about the factors that modify enzyme activity.  Using a turnip extract containing the enzyme peroxidase, we will demonstrate the effects that modulators—such as temperature, pH, and inhibitors—have on this enzyme.  

Unit 2 Time Advisory
This unit will take approximately 4 hours to complete.

  • Web Media: YouTube: brightstorm’s “Enzymes” Link: YouTube: brightstorm’s “Enzymes" (YouTube)

    Instructions: Please watch this video clip (about 4 minutes) for an overview of the enzymatic reactions and the factors that affect these reactions.

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Web Media: YouTube: WPI: “Enzyme Activity Using a Turnip” and MissyClever: “Enzyme Lab: Temperature” and “Enzyme Activity: pH” Links: WPI: “Enzyme Activity Using a Turnip” (YouTube) and MissyClever: “Enzyme Lab: Temperature” (YouTube) and “Enzyme Activity: pH” (YouTube)
     
    Instructions: Please review these three video clips (about 10 minutes in total) to watch the step-by-step procedure of this experiment, including how to prepare the turnip extract and how to measure the effects of different temperatures and pH levels on the enzyme activity.  You will find the experiment’s objectives and procedures in the next resource box. 
     
    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.

  • Activity: Purdue University: “Factors Affecting Enzyme Activity”

     Link: Purdue University: “Factors Affecting Enzyme Activity” (PDF) 

    Instructions:  Please review this activity to learn about the objectives and the procedures of the experiment you watched above.  Then, based on your knowledge of the enzymes and their modified activity by modulators, please record what results you would expect when conducting the temperature, pH, and inhibitor experiments.

    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.  

  • Activity: University of Illinois, Chicago: BIOS100 Lab: “Determination of the Properties of the Enzyme Turnip Peroxidase” Link: University of Illinois, Chicago: BIOS100 Lab: “Determination of the Properties of the Enzyme Turnip Peroxidase” (HTML)
     
    Instructions: Please use this resource to check your results from the “Factors Affecting Enzyme Activity” exercise. 
     
    Terms of Use: Please respect the copyright and terms of use displayed on the webpage above.